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Abstract: Neem (Azadirachta indica Juss.) is a plant belonging to the Meliaceae family. Neem leaves contain active compounds of flavonoids, saponins, tannins, β-sitosterol, nimbolide, quercetin, routine, and nimbine. which has the potential to heal wounds. This research was conducted to determine the effect of giving crushed neem leaves (DMT) on the mean wound shrinkage in zebrafish. This research was a real laboratory experimental study, using a completely randomized design with 4 treatments and 7 replications. 28 zebrafish were divided into 4 groups and injured 0.3 cm in length. Group 1 as a control (without DMT), groups 2, 3, and 4 as treatment with DMT concentrations of 1 gram (10%), 2 grams (20%), and 3 grams (30%). Data were analyzed using the Analysis Of Variance (ANOVA) test with a confidence level of 95%. The results were significantly different (p<0.05) followed by further Tukey post hoc test. Based on the results of the ANOVA test, the value of p <0.001 shows that there is a significant difference (p<0.05) between the control and treatment. The control group was significantly different from the P1, P2, and P3 groups. The provision of DMT was proven to have a significant effect on the shrinkage of zebrafish wounds. Wound shrinkage in zebrafish occurs due to the presence of flavonoids, saponins, and tannins which function as antibacterial, anti-inflammatory, and astringent compounds that shrink skin pores and prevent edema. DMT affects the wound healing phase, the inflammatory stage, cell proliferation, matrix deposition to the remodeling phase.

Keywords: Wound healing, Zebrafish; Neem leaves

Abstrak: Mimba (Azadirachta indica Juss.) adalah tanaman yang termasuk dalam suku meliaceae. Daun mimba mengandung senyawa aktif flavonoid, saponin, tannin, β-sitosterol, nimbolide, quercetin, rutin, dan nimbine. yang berpotensi untuk menyembuhkan luka. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian daun mimba tumbuk (DMT) terhadap rerata pengerutan luka pada ikan zebra. Penelitian ini bersifat eksperimental laboratorik sungguhan, menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 7 ulangan. Ikan zebra sebanyak 28 ekor dibagi dalam 4 kelompok dan dilukai gurat sisinya sepanjang 0,3 cm. Kelompok 1 sebagai kontrol (tanpa DMT), kelompok 2, 3 dan 4 sebagai perlakuan dengan konsentrasi DMT 1 gram (10%), 2 gram (20%) dan 3 gram (30%). Data dianalisis menggunakan uji Analisis Of Varians (ANOVA) dengan tingkat kepercayaan 95%. Hasil berbeda nyata (p<0.05) dilanjutkan dengan uji lanjut Tukey post hoc test. Berdasarkan hasil uji ANOVA didapatkan nilai p<0.001 menunjukkan bahwa terdapat perbedaan yang bermakna (p<0,05) antara kontrol dan perlakuan. Kelompok kontrol berbeda signifikan dengan kelompok P1, P2 dan P3. Pemberian DMT terbukti berpengaruh nyata terhadap pengerutan luka ikan zebra. Pengerutan luka pada ikan zebra terjadi karena adanya senyawa flavonoid, saponin, dan tanin yang berfungsi sebagai senyawa antibakteri, anti imflamasi dan astringen yang mengecilkan pori-pori kulit dan mencegah edema. DMT memengaruhi fase penyembuhan luka, pada tahap inflamasi, proliferasi sel, deposisi matriks hingga fase remodeling.

Kata kunci: Penyembuhan luka, ikan zebra, daun mimba Neem (Azadirachta indica Juss.) is a plant belonging to the Meliaceae family. Neem leaves contain active compounds of flavonoids, saponins, tannins, β-sitosterol, nimbolide, quercetin, routine, and nimbine. which has the potential to heal wounds. This research was conducted to determine the effect of giving crushed neem leaves (DMT) on the mean wound shrinkage in zebrafish. This research was a real laboratory experimental study, using a completely randomized design with 4 treatments and 7 replications. 28 zebrafish were divided into 4 groups and injured 0.3 cm in length. Group 1 as a control (without DMT), groups 2, 3, and 4 as treatment with DMT concentrations of 1 gram (10%), 2 grams (20%), and 3 grams (30%). Data were analyzed using the Analysis Of Variance (ANOVA) test with a confidence level of 95%. The results were significantly different (p<0.05) followed by further Tukey post hoc test. Based on the results of the ANOVA test, the value of p <0.001 shows that there is a significant difference (p<0.05) between the control and treatment. The control group was significantly different from the P1, P2, and P3 groups. The provision of DMT was proven to have a significant effect on the shrinkage of zebrafish wounds. Wound shrinkage in zebrafish occurs due to the presence of flavonoids, saponins, and tannins which function as antibacterial, anti-inflammatory, and astringent compounds that shrink skin pores and prevent edema. DMT affects the wound healing phase, the inflammatory stage, cell proliferation, matrix deposition to the remodeling phase.